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class="title"><h3>不是速离心机太神U,而是你不了解?/h3></div> <div class="infos">发布日期Q?018/11/21 17:18:10      讉Kơ数: <script language="javascript" src="/inc/article_view.asp?id=1348"></script></div> <div class="maincontent clearfix">   不是速离心机太神U,而是你不了解?br />   <strong><a href="http://www.oohlalala.com/Product/20130916335.html" target="_blank">速离心机</a></strong>是生物医学实验室和医院最常用的科研A器之一 Q它可以通过高速旋转生的d力场对具有不同沉降系?、质量和密度的؜合物q行快速分?、浓~和U化 ?速离心机作ؓ一U常用的实验手段 Q具有许多优??例如可在低温下进行离?Q这保证了生物大分子的活性不被破?Q制备型速离心机的负载量?Q一ơ可分离提纯几克样品 Q远大于层析和电泳的上样量?因此速离心技术在d 、生命科学等相关专业研究中占有重要地?Q是亚细胞组份、蛋白质、RNA 和质_?DNA {的最方便高效的实验技术之一?br />   (1)甉|开?Q打开甉| Q待d机的昄器显C出正常的操作界?Q?br />   (2)装入样品 Q将装有样品的离心管攑օ相应的角转子中或水^转子的吊桶中 Q将吊桶悬挂在水q{子上 Q?br />   (3)攄转子 Q双手握紧{子边~将转子提v Q对准离心机转u轻轻攄 Q确认放好后 Q关上仓?Q?br />   (4)讄参数 Q在昄器操作界面上选择所用{子的型号 Q离心{?Q离心时间及d温度 Q按“Download”键认q回C界面 Q?br />   (5)开始离?Q在ȝ面上按“Precool”键预冷 Q当界面昄的温度达到设定温度后 Q按“Ente”键和“Start”键开始离?Q?br />   (6)dl束 Q当d鸣器提示dl束?Q按“Vacuum”键使离心仓从真I状态恢复到正常气压 Q?br />   (7)攉样品 Q打开仓门 Q取{子和d?Q再从离心管中收集目标区?Q?br />   (8)关闭d?Q用软布擦拭转子和吊?Q关上仓?Q最后关闭电?Q?br />   (9)登记信息 Q在专用登记本上记录本次dZ用的相关信息 ?br />   <strong>速离心机</strong>工作速度范围q泛Q可处理多种样品Q是U研实验中不可或~的实验手段Q广泛应用于生物大分子、细胞器、细胞、病毒等的分ȝ化。经dU化可直接获得有关细胞或l胞器、病毒、生物大分子的相关信息,一步研I其生物学特性奠定基。离心分LU某U颗_时Q可参考前人的l验Q同ӞҎ要分颗_的性质和所h的离心条件及提纯要求选用不同的离心方案。在设计dҎӞ首先选择合适的梯度材料Q应考虑其溶液的最大密度范围是否合适,所选用的物质是否媄响样品的zL,对{头和d是否有腐蚀作用{。其ơ选择合适的d方式Q由于生物颗_的沉降pL有一定的范围Q有时可Ҏ其沉降系数或密度来分离某种颗粒Q但Z得到最好的效果Q往往必须同时考虑q两个特性,l合不同的离心技术进行分R最后选择合适的转头Q差速离心选用角{头最有效Q速率区带d最好用水q{_{密度梯度离心可以用角{头或者水q{头。值得注意的是Q{头对重盐梯度的最大允讔R度Q由于{头的最大速度是根据离心管所装溶液的密度计算的,如果该溶液密度超q此速度Q则应相应降低最大允讔R度。不同{头的最大允讔R度及具体减速数值在转头说明书中皆有图表说明。MQ在使用速离心机Ӟ除了设计U学合理的离心方案,q应严格按说明操作,q定期维护离心机及其部gQ以充分发挥<strong>?/strong><strong>d?/strong>的利用效?满相关专业学科的实验需求? </div> <div class="prenext"> <ul><li>上一: 没有?/li><li>下一:<a href='/html/8540934944.html' target='_blank' title='惌研制d机,先来了解d机的发展状况吧!'>惌研制d机,先来了解d机的发展状况吧!</a> <span class='ListDate'>2018/11/16</span></li></ul> </div> </div> <!--content end--> </div> <!--main end--> </div> <!--right end--> </div> <!--inner end--> <div 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